2KXE

N-terminal domain of the DP1 subunit of an archaeal D-family DNA polymerase

2KXE の概要

• エントリーDOI: 10.2210/pdb2kxe/pdb
• NMR情報: BMRB: 16986
• 分子名称: DNA polymerase II small subunit (1 entity in total)
• 機能のキーワード: dna polymerase, d-family, small subunit, archara, helical bundle, transferase
• 由来する生物種: Pyrococcus horikoshii
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8534.80

構造登録者

Yamasaki, K.,Matsui, I. (登録日: 2010-04-30, 公開日: 2010-08-18, 最終更新日: 2024-05-15)

主引用文献

Yamasaki, K.,Urushibata, Y.,Yamasaki, T.,Arisaka, F.,Matsui, I.
Solution structure of the N-terminal domain of the archaeal D-family DNA polymerase small subunit reveals evolutionary relationship to eukaryotic B-family polymerases
Febs Lett., 584:3370-3375, 2010

PubMed Abstract: Archaea-specific D-family DNA polymerase forms a heterotetramer consisting of two large polymerase subunits and two small exonuclease subunits. We analyzed the structure of the N-terminal 200 amino-acid regulatory region of the small subunit by NMR and revealed that the N-terminal approximately 70 amino-acid region is folded. The structure consists of a four-alpha-helix bundle including a short parallel beta-sheet, which is similar to the N-terminal regions of the B subunits of human DNA polymerases alpha and epsilon, establishing evolutionary relationships among these archaeal and eukaryotic polymerases. We observed monomer-dimer equilibrium of this domain, which may be related to holoenzyme architecture and/or functional regulation.

PubMed: 20598295
DOI: 10.1016/j.febslet.2010.06.026

実験手法

SOLUTION NMR