2KVQ

Solution structure of NusE:NusG-CTD complex

2KVQ の概要

• エントリーDOI: 10.2210/pdb2kvq/pdb
• NMR情報: BMRB: 16788
• 分子名称: NusE, Transcription antitermination protein nusG (2 entities in total)
• 機能のキーワード: nuse:nusg complex, transcription
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 16563.98

構造登録者

Burmann, B.M.,Schweimer, K.,Roesch, P. (登録日: 2010-03-25, 公開日: 2010-05-05, 最終更新日: 2024-05-22)

主引用文献

Burmann, B.M.,Schweimer, K.,Luo, X.,Wahl, M.C.,Stitt, B.L.,Gottesman, M.E.,Rosch, P.
A NusE:NusG complex links transcription and translation.
Science, 328:501-504, 2010

PubMed Abstract: Bacterial NusG is a highly conserved transcription factor that is required for most Rho activity in vivo. We show by nuclear magnetic resonance spectroscopy that Escherichia coli NusG carboxyl-terminal domain forms a complex alternatively with Rho or with transcription factor NusE, a protein identical to 30S ribosomal protein S10. Because NusG amino-terminal domain contacts RNA polymerase and the NusG carboxy-terminal domain interaction site of NusE is accessible in the ribosomal 30S subunit, NusG may act as a link between transcription and translation. Uncoupling of transcription and translation at the ends of bacterial operons enables transcription termination by Rho factor, and competition between ribosomal NusE and Rho for NusG helps to explain why Rho cannot terminate translated transcripts.

PubMed: 20413501
DOI: 10.1126/science.1184953

実験手法

SOLUTION NMR