2KVN

Phi29 E-loop hairpin

Summary for 2KVN

• Entry DOI: 10.2210/pdb2kvn/pdb
• Related: 1HS2
• Descriptor: RNA (5'-R(*GP*GP*UP*GP*AP*UP*UP*GP*AP*GP*UP*UP*CP*AP*CP*CP*A)-3') (1 entity in total)
• Functional Keywords: phi29, hairpin, rna
• Total number of polymer chains: 1
• Total formula weight: 5444.27

Authors

Harris, S.M.,Schroeder, S.J. (deposition date: 2010-03-19, release date: 2010-07-07, Last modification date: 2024-05-01)

Primary citation

Harris, S.,Schroeder, S.J.
Nuclear magnetic resonance structure of the prohead RNA E-loop hairpin.
Biochemistry, 49:5989-5997, 2010

PubMed Abstract: The Bacillus subtilis phage phi29 packaging motor requires prohead RNA for genome encapsidation. The nuclear magnetic resonance structure of the prohead RNA E-loop hairpin, r(5'AUUGAGUU), is presented and compared to predictions from MC-SYM. The prohead RNA E-loop hairpins contain sequences similar to rRNA hairpins. Comparison of predicted and experimentally determined prohead and ribosomal hairpin structures reveals that sequence similarity is a stronger determinant of hairpin structural similarity than grouping similar types of RNA. All the hairpins contain a U-turn motif but differ in the first noncanonical pair and backbone orientation. These structures provide benchmarks for further improvements in RNA structure predictions from sequence.

PubMed: 20550192
DOI: 10.1021/bi100393r

Experimental method

SOLUTION NMR