2KV2

Solution Structure of the human BLM HRDC domain

2KV2 の概要

• エントリーDOI: 10.2210/pdb2kv2/pdb
• 分子名称: Bloom syndrome protein (1 entity in total)
• 機能のキーワード: bloom syndrome, hrdc domain, disease mutation, dna replication, dna-binding, nucleotide-binding, nucleus, gene regulation
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus: P54132
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9594.92

構造登録者

Kim, Y.M.,Choi, B.-S. (登録日: 2010-03-04, 公開日: 2010-12-15, 最終更新日: 2024-05-01)

主引用文献

Kim, Y.M.,Choi, B.-S.
Structure and function of the regulatory HRDC domain from human Bloom syndrome protein
Nucleic Acids Res., 38:7764-7777, 2010

PubMed Abstract: The helicase and RNaseD C-terminal (HRDC) domain, conserved among members of the RecQ helicase family, regulates helicase activity by virtue of variations in its surface residues. The HRDC domain of Bloom syndrome protein (BLM) is known as a critical determinant of the dissolution function of double Holliday junctions by the BLM-Topoisomerase IIIα complex. In this study, we determined the solution structure of the human BLM HRDC domain and characterized its DNA-binding activity. The BLM HRDC domain consists of five α-helices with a hydrophobic 3(10)-helical loop between helices 1 and 2 and an extended acidic surface comprising residues in helices 3-5. The BLM HRDC domain preferentially binds to ssDNA, though with a markedly low binding affinity (K(d) ∼100 μM). NMR chemical shift perturbation studies suggested that the critical DNA-binding residues of the BLM HRDC domain are located in the hydrophobic loop and the N-terminus of helix 2. Interestingly, the isolated BLM HRDC domain had quite different DNA-binding modes between ssDNA and Holliday junctions in electrophoretic mobility shift assay experiments. Based on its surface charge separation and DNA-binding properties, we suggest that the HRDC domain of BLM may be adapted for a unique function among RecQ helicases--that of bridging protein and DNA interactions.

PubMed: 20639533
DOI: 10.1093/nar/gkq586

実験手法

SOLUTION NMR