2KUU

Solution Structure of K10 TLS RNA (GC mutant in upper helix)

Summary for 2KUU

• Entry DOI: 10.2210/pdb2kuu/pdb
• Related: 2KE6 2KUR 2KUV 2KUW
• Descriptor: K10 TLS RNA (1 entity in total)
• Functional Keywords: rna transport, rna hairpin, rna
• Biological source: Drosophila melanogaster (Fruit fly)
• Total number of polymer chains: 1
• Total formula weight: 15285.05

Authors

Bullock, S.L.,Ringel, I.,Ish-Horowicz, D.,Lukavsky, P.J. (deposition date: 2010-03-01, release date: 2010-05-19, Last modification date: 2024-05-22)

Primary citation

Bullock, S.L.,Ringel, I.,Ish-Horowicz, D.,Lukavsky, P.J.
A'-form RNA helices are required for cytoplasmic mRNA transport in Drosophila.
Nat.Struct.Mol.Biol., 17:703-709, 2010

PubMed Abstract: Microtubule-based mRNA transport is widely used to restrict protein expression to specific regions in the cell and has important roles in defining cell polarity and axis determination as well as in neuronal function. However, the structural basis of recognition of cis-acting mRNA localization signals by motor complexes is poorly understood. We have used NMR spectroscopy to describe the first tertiary structure to our knowledge of an RNA element responsible for mRNA transport. The Drosophila melanogaster fs(1)K10 signal, which mediates transport by the dynein motor, forms a stem loop with two double-stranded RNA helices adopting an unusual A'-form conformation with widened major grooves reminiscent of those in B-form DNA. Structure determination of four mutant RNAs and extensive functional assays in Drosophila embryos indicate that the two spatially registered A'-form helices represent critical recognition sites for the transport machinery. Our study provides insights into the basis for RNA cargo recognition and reveals a key biological function encoded by A'-form RNA conformation.

PubMed: 20473315
DOI: 10.1038/nsmb.1813

Experimental method

SOLUTION NMR