2KU2

Dynamic Regulation of Archaeal Proteasome Gate Opening as Studied by TROSY-NMR

2KU2 の概要

• エントリーDOI: 10.2210/pdb2ku2/pdb
• 関連するPDBエントリー: 1PMA 1YA7 2KU1
• 分子名称: Proteasome subunit alpha (1 entity in total)
• 機能のキーワード: proteasome, proteolysis, gating residues, cytoplasm, hydrolase, protease, threonine protease
• 由来する生物種: Thermoplasma acidophilum
• 細胞内の位置: Cytoplasm (By similarity): P25156
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 181871.66

構造登録者

Religa, T.L.,Sprangers, R.,Kay, L.E. (登録日: 2010-02-11, 公開日: 2010-04-21, 最終更新日: 2024-05-22)

主引用文献

Religa, T.L.,Sprangers, R.,Kay, L.E.
Dynamic regulation of archaeal proteasome gate opening as studied by TROSY NMR.
Science, 328:98-102, 2010

PubMed Abstract: The proteasome catalyzes the majority of protein degradation in the cell and plays an integral role in cellular homeostasis. Control over proteolysis by the 20S core-particle (CP) proteasome is achieved by gated access of substrate; thus, an understanding of the molecular mechanism by which these gates regulate substrate entry is critical. We used methyl-transverse relaxation optimized nuclear magnetic resonance spectroscopy to show that the amino-terminal residues that compose the gates of the alpha subunits of the Thermoplasma acidophilum proteasome are highly dynamic over a broad spectrum of time scales and that gating termini are in conformations that extend either well inside (closed gate) or outside (open gate) of the antechamber. Interconversion between these conformers on a time scale of seconds leads to a dynamic regulation of 20S CP proteolysis activity.

PubMed: 20360109
DOI: 10.1126/science.1184991

実験手法

SOLUTION NMR