2KTC

Solution Structure of a Novel hKv1.1 inhibiting scorpion toxin from Mesibuthus tamulus

2KTC の概要

• エントリーDOI: 10.2210/pdb2ktc/pdb
• NMR情報: BMRB: 16693
• 分子名称: Potassium channel toxin alpha-KTx 9.4 (1 entity in total)
• 機能のキーワード: btk-2, scorpion toxin, kv1.1, disulfide bond, ionic channel inhibitor, neurotoxin, potassium channel inhibitor, secreted, toxin
• 由来する生物種: Mesobuthus tamulus (eastern Indian scorpion)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 3607.32

構造登録者

Kumar, G.S.,Upadhyay, S.,Mathew, M.K.,Sarma, S.P. (登録日: 2010-01-26, 公開日: 2011-02-02, 最終更新日: 2024-11-06)

主引用文献

Kumar, G.S.,Upadhyay, S.,Mathew, M.K.,Sarma, S.P.
Solution structure of BTK-2, a novel hK(v)1.1 inhibiting scorpion toxin, from the eastern Indian scorpion Mesobuthus tamulus.
Biochim.Biophys.Acta, 1814:459-469, 2011

PubMed Abstract: The three dimensional structure of a 32 residue three disulfide scorpion toxin, BTK-2, from the Indian red scorpion Mesobuthus tamulus has been determined using isotope edited solution NMR methods. Samples for structural and electrophysiological studies were prepared using recombinant DNA methods. Electrophysiological studies show that the peptide is active against hK(v)1.1 channels. The structure of BTK-2 was determined using 373 distance restraints from NOE data, 66 dihedral angle restraints from NOE, chemical shift and scalar coupling data, 6 constraints based on disulfide linkages and 8 constraints based on hydrogen bonds. The root mean square deviation (r.m.s.d) about the averaged co-ordinates of the backbone (N, C(α), C') and all heavy atoms are 0.81 ± 0.23Å and 1.51 ± 0.29Å respectively. The backbone dihedral angles (ϕ and ψ) for all residues occupy the favorable and allowed regions of the Ramachandran map. The three dimensional structure of BTK-2 is composed of three well defined secondary structural regions that constitute the α-β-β structural motif. Comparisons between the structure of BTK-2 and other closely related scorpion toxins pointed towards distinct differences in surface properties that provide insights into the structure-function relationships among this important class of voltage-gated potassium channel inhibiting peptides.

PubMed: 21256986
DOI: 10.1016/j.bbapap.2011.01.006

実験手法

SOLUTION NMR