2KT4

Lipocalin Q83 is a Siderocalin

2KT4 の概要

• エントリーDOI: 10.2210/pdb2kt4/pdb
• NMR情報: BMRB: 16682
• 分子名称: Extracellular fatty acid-binding protein, N,N',N''-[(3S,7S,11S)-2,6,10-trioxo-1,5,9-trioxacyclododecane-3,7,11-triyl]tris(2,3-dihydroxybenzamide), GALLIUM (III) ION (3 entities in total)
• 機能のキーワード: siderocalin, lipocalin, enterobactin, disulfide bond, secreted, transport, transport protein
• 由来する生物種: Coturnix japonica (Japanese quail)
• 細胞内の位置: Secreted : Q9I9P7
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 18844.00

構造登録者

Coudevylle, N.,Geist, L.,Hartl, M.,Kontaxis, G.,Bister, K.,Konrat, R. (登録日: 2010-01-18, 公開日: 2010-09-08, 最終更新日: 2024-11-27)

主引用文献

Coudevylle, N.,Geist, L.,Hotzinger, M.,Hartl, M.,Kontaxis, G.,Bister, K.,Konrat, R.
The v-myc-induced Q83 lipocalin is a siderocalin.
J.Biol.Chem., 285:41646-41652, 2010

PubMed Abstract: Siderocalins are atypical lipocalins able to capture siderophores with high affinity. They contribute to the innate immune response by interfering with bacterial siderophore-mediated iron uptake but are also involved in numerous physiological processes such as inflammation, iron delivery, tissue differentiation, and cancer progression. The Q83 lipocalin was originally identified based on its overexpression in quail embryo fibroblasts transformed by the v-myc oncogene. We show here that Q83 is a siderocalin, binding the siderophore enterobactin with an affinity and mode of binding nearly identical to that of neutrophil gelatinase-associated lipocalin (NGAL), the prototypical siderocalin. This strengthens the role of siderocalins in cancer progression and inflammation. In addition, we also present the solution structure of Q83 in complex with intact enterobactin and a detailed analysis of the Q83 binding mode, including mutagenesis of the critical residues involved in enterobactin binding. These data provide a first insight into the molecular details of siderophore binding and delineate the common molecular properties defining the siderocalin protein family.

PubMed: 20826777
DOI: 10.1074/jbc.M110.123331

実験手法

SOLUTION NMR