2KSK
Solution Structure of Sugarcane defensin 5
Summary for 2KSK
| Entry DOI | 10.2210/pdb2ksk/pdb |
| Descriptor | Sugarcane defensin 5 (1 entity in total) |
| Functional Keywords | csalphabeta motif, antimicrobial protein |
| Biological source | Saccharum officinarum |
| Total number of polymer chains | 1 |
| Total formula weight | 7981.21 |
| Authors | de Paula, V.S.,Almeida, F.C.L.,Valente, A. (deposition date: 2010-01-06, release date: 2011-01-26, Last modification date: 2024-10-09) |
| Primary citation | de Paula, V.S.,Razzera, G.,Barreto-Bergter, E.,Almeida, F.C.,Valente, A.P. Portrayal of Complex Dynamic Properties of Sugarcane Defensin 5 by NMR: Multiple Motions Associated with Membrane Interaction. Structure, 19:26-36, 2011 Cited by PubMed Abstract: Defensins are essentially ancient natural antibiotics with potent activity extending from lower organisms to humans. Sd5 is a recently described antifungal defensin that appears to be the result of a recent gain of function. We reported here the solution NMR structure of Sd5 and characterized the backbone dynamics in the free state and in the presence of membrane models. (15)N relaxation dispersion measurements indicate intrinsic conformational exchange processes, showing two clear distinct k(ex), 490 and 1800 s(-1). These multiple motions may be related to transient twisting or breathing of the α helix and β sheet. The stages of membrane recognition and disruption by Sd5 over a large timescale range were mapped and demonstrated that Sd5 in solution sampled an ensemble of different conformations, of which a subset is selected upon membrane binding. Defensins share similar structures, but we demonstrated here that their dynamics can be extremely diverse. PubMed: 21220113DOI: 10.1016/j.str.2010.11.011 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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