2KQS

Phosphorylation of SUMO-interacting motif by CK2 enhances Daxx SUMO binding activity

2KQS の概要

• エントリーDOI: 10.2210/pdb2kqs/pdb
• 関連するPDBエントリー: 2ASQ 2RPQ
• 分子名称: Small ubiquitin-related modifier 1, Death domain-associated protein 6 (2 entities in total)
• 機能のキーワード: sumo, sim, daxx, nucleus, phosphoprotein, ubl conjugation pathway, apoptosis, transcription, transcription regulation
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus membrane: P63165; Cytoplasm: Q9UER7
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 13589.13

構造登録者

Naik, M.T.,Huang, T.H.,Shih, H. (登録日: 2009-11-17, 公開日: 2010-12-01, 最終更新日: 2024-05-01)

主引用文献

Chang, C.C.,Naik, M.T.,Huang, Y.S.,Jeng, J.C.,Liao, P.H.,Kuo, H.Y.,Ho, C.C.,Hsieh, Y.L.,Lin, C.H.,Huang, N.J.,Naik, N.M.,Kung, C.C.,Lin, S.Y.,Chen, R.H.,Chang, K.S.,Huang, T.H.,Shih, H.M.
Structural and functional roles of Daxx SIM phosphorylation in SUMO paralog-selective binding and apoptosis modulation.
Mol.Cell, 42:62-74, 2011

PubMed Abstract: Small ubiquitin-like modifier (SUMO) conjugation and interaction are increasingly associated with various cellular processes. However, little is known about the cellular signaling mechanisms that regulate proteins for distinct SUMO paralog conjugation and interactions. Using the transcriptional coregulator Daxx as a model, we show that SUMO paralog-selective binding and conjugation are regulated by phosphorylation of the Daxx SUMO-interacting motif (SIM). NMR structural studies show that Daxx (732)E-I-I-V-L-S-D-S-D(740) is a bona fide SIM that binds to SUMO-1 in a parallel orientation. Daxx-SIM is phosphorylated by CK2 kinase at residues S737 and S739. Phosphorylation promotes Daxx-SIM binding affinity toward SUMO-1 over SUMO-2/3, causing Daxx preference for SUMO-1 conjugation and interaction with SUMO-1-modified factors. Furthermore, Daxx-SIM phosphorylation enhances Daxx to sensitize stress-induced cell apoptosis via antiapoptotic gene repression. Our findings provide structural insights into the Daxx-SIM:SUMO-1 complex, a model of SIM phosphorylation-enhanced SUMO paralog-selective modification and interaction, and phosphorylation-regulated Daxx function in apoptosis.

PubMed: 21474068
DOI: 10.1016/j.molcel.2011.02.022

実験手法

SOLUTION NMR