2KPC

Structure determination of the top-loop of the conserved 3 terminal secondary structure in the genome of YFV

2KPC の概要

• エントリーDOI: 10.2210/pdb2kpc/pdb
• 関連するPDBエントリー: 2KPD
• 分子名称: RNA (5'-R(*UP*GP*AP*GP*CP*AP*CP*AP*GP*UP*UP*UP*GP*CP*UP*CP*A)-3') (1 entity in total)
• 機能のキーワード: yellow fever virus, 3'-terminal loop, genome, rna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 5404.24

構造登録者

Lescrinier, E.,Dyubankova, N.,Nauwelaerts, K.,Jones, R.,Herdewijn, P. (登録日: 2009-10-12, 公開日: 2010-06-30, 最終更新日: 2024-05-22)

主引用文献

Lescrinier, E.,Dyubankova, N.,Nauwelaerts, K.,Jones, R.,Herdewijn, P.
Structure Determination of the Top-Loop of the Conserved 3'-Terminal Secondary Structure in the Genome of Flaviviruses.
Chembiochem, 11:1404-1412, 2010

PubMed Abstract: To what extent small differences in RNA sequences (mutations) can have a profound impact on biology remains an intriguing question. This effect can be studied by using untranslated RNA regions as a model. We have studied the influence of mutations on the structure of an RNA hairpin that occurs in the 3'-untranslated region (UTR) of Flaviviridae, and is known to have a large impact on the vector dependency of flaviviruses. Three related RNA sequences were studied by NMR spectroscopy. The selected sequences represent each one of the three clusters in the flavivirus genes (mosquito-borne, tick-borne, and no-known-vector viruses). A new strategy was used to obtain chemical shift signatures of carbonyl atoms in unlabeled uridine nucleobases to characterize their involvement in hydrogen bonding. Clear differences occur in the structures and stacking pattern of the three RNA hairpins. The observed differences cannot be predicted based on sequence analysis. A different biology can be correlated with a different RNA tertiary structure. The underlying biological mechanism, however, remains to be studied.

PubMed: 20540055
DOI: 10.1002/cbic.200900765

実験手法

SOLUTION NMR