2KO3

Nedd8 solution structure

2KO3 の概要

• エントリーDOI: 10.2210/pdb2ko3/pdb
• 分子名称: NEDD8 (1 entity in total)
• 機能のキーワード: nedd8, isopeptide bond, nucleus, ubl conjugation pathway, signaling protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus: Q15843
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8573.98

構造登録者

Choi, Y.S.,Jeon, Y.H.,Cheong, C. (登録日: 2009-09-09, 公開日: 2009-11-03, 最終更新日: 2024-05-29)

主引用文献

Choi, Y.S.,Jeon, Y.H.,Ryu, K.S.,Cheong, C.
60th residues of ubiquitin and Nedd8 are located out of E2-binding surfaces, but are important for K48 ubiquitin-linkage.
Febs Lett., 583:3323-3328, 2009

PubMed Abstract: Nedd8, a ubiquitin-like modifier, is covalently attached to various proteins. Although Nedd8 has higher sequence identity (57%) with ubiquitin, its conserved K48 residue cannot form covalent linkage with ubiquitin. To decipher the reason why Nedd8 cannot be an effective ubiquitin-acceptor, we compared the non-covalent interaction between Nedd8 and ubiquitin for various E2s using cross-saturation NMR technique. However, both Nedd8 and ubiquitin displayed almost identical non-covalent E2-binding properties. The K60 of Nedd8 was not present at the E2-binding surface, but its mutation to Asn converted Nedd8 into a ubiquitin-acceptor. The N60 ubiquitin mutants also displayed a decreased ubiquitin-accepting activity. These results suggest the presence of an uncharacterized determinant for the K48 ubiquitin-linkage that is not related to non-covalent E2-bindings.

PubMed: 19782077
DOI: 10.1016/j.febslet.2009.09.034

実験手法

SOLUTION NMR