2KN7

Structure of the XPF-single strand DNA complex

2KN7 の概要

• エントリーDOI: 10.2210/pdb2kn7/pdb
• 関連するPDBエントリー: 1z00 2aq0
• NMR情報: BMRB: 16449
• 分子名称: DNA repair endonuclease XPF, DNA (5'-D(*CP*AP*GP*TP*GP*GP*CP*TP*GP*A)-3') (2 entities in total)
• 機能のキーワード: ner, xpf/ercc1, hhh, protein-ssdna complex, hydrolase-dna complex, hydrolase/dna
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus (Probable): Q92889
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 21016.94

構造登録者

Das, D.,Folkers, G.E.,van Dijk, M.,Jaspers, N.G.J.,Hoeijmakers, J.H.J.,Kaptein, R.,Boelens, R. (登録日: 2009-08-16, 公開日: 2010-08-04, 最終更新日: 2024-05-01)

主引用文献

Das, D.,Folkers, G.E.,van Dijk, M.,Jaspers, N.G.J.,Hoeijmakers, J.H.J.,Kaptein, R.,Boelens, R.
The structure of the XPF-ssDNA complex underscores the distinct roles of the XPF and ERCC1 helix- hairpin-helix domains in ss/ds DNA recognition
Structure, 20:667-675, 2012

PubMed Abstract: Human XPF/ERCC1 is a structure-specific DNA endonuclease that nicks the damaged DNA strand at the 5' end during nucleotide excision repair. We determined the structure of the complex of the C-terminal domain of XPF with 10 nt ssDNA. A positively charged region within the second helix of the first HhH motif contacts the ssDNA phosphate backbone. One guanine base is flipped out of register and positioned in a pocket contacting residues from both HhH motifs of XPF. Comparison to other HhH-containing proteins indicates a one-residue deletion in the second HhH motif of XPF that has altered the hairpin conformation, thereby permitting ssDNA interactions. Previous nuclear magnetic resonance studies showed that ERCC1 in the XPF-ERCC1 heterodimer can bind dsDNA. Combining the two observations gives a model that underscores the asymmetry of the human XPF/ERCC1 heterodimer in binding at an ss/ds DNA junction.

PubMed: 22483113
DOI: 10.1016/j.str.2012.02.009

実験手法

SOLUTION NMR