2KMK

Gfi-1 Zinc Fingers 3-5 complexed with DNA

2KMK の概要

• エントリーDOI: 10.2210/pdb2kmk/pdb
• 分子名称: Zinc finger protein Gfi-1, DNA (5'-D(*CP*AP*TP*AP*AP*AP*TP*CP*AP*CP*TP*GP*CP*CP*TP*A)-3'), DNA (5'-D(*TP*AP*GP*GP*CP*AP*GP*TP*GP*AP*TP*TP*TP*AP*TP*G)-3'), ... (4 entities in total)
• 機能のキーワード: tandem repeat zinc finger domain, protein-dna complex, dna-binding, metal-binding, nucleus, transcription, transcription regulation, zinc, zinc-finger, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Rattus norvegicus (brown rat,rat,rats)
• 細胞内の位置: Nucleus: Q07120
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 19394.50

構造登録者

Lee, S.,Wu, Z. (登録日: 2009-07-30, 公開日: 2010-03-02, 最終更新日: 2024-05-22)

主引用文献

Lee, S.,Doddapaneni, K.,Hogue, A.,McGhee, L.,Meyers, S.,Wu, Z.
Solution structure of Gfi-1 zinc domain bound to consensus DNA.
J.Mol.Biol., 397:1055-1066, 2010

PubMed Abstract: Gfi-1 is a crucial transcriptional repressor for the precise regulation of cell proliferation and differentiation in hematopoiesis. Recently, this protein has also been demonstrated to be capable of restricting the proliferation of hematopoietic stem cells, a process that appears to be vital for the long-term competency of hematopoietic stem cells. These two seemingly opposite outcomes of regulation are likely to arise from its interactions with a variety of cellular partners. Such interactions can directly affect the genes that Gfi-1 recognizes through its DNA binding zinc-finger domain. In this work, we report the determination of the solution structure of Gfi-1 zinc fingers 3-5 in complex with a 16-mer consensus DNA using multidimensional NMR method. Unlike a proposed minor-groove binding model based on methylation interference experiments, our structure clearly shows that Gfi-1 zinc fingers 3-5 bind into the major groove of the target DNA reminiscent of canonical C(2)H(2) zinc-finger domains. The fourth and fifth zinc fingers recognize the AATC core sequence by forming base-specific hydrogen bonds between the side chains of Asn382, Gln379, and Asp354 and the bases of the invariant adenines and cytosine. Overall, the current work provides valuable insight into the structural determinants for DNA binding specificity, in particular for the TCA triplet that has not been observed in any other structures of zinc finger-DNA complexes, as well as molecular rationales for a naturally occurring mutation that causes acute myeloid leukemia.

PubMed: 20153336
DOI: 10.1016/j.jmb.2010.02.006

実験手法

SOLUTION NMR