2KLV

Membrane-bound structure of the Pf1 major coat protein in DHPC micelle

2KLV の概要

• エントリーDOI: 10.2210/pdb2klv/pdb
• 関連するPDBエントリー: 1PJF
• 分子名称: Capsid protein G8P (1 entity in total)
• 機能のキーワード: membrane protein, alignment, alpha helix, capsid protein, membrane, transmembrane, virion
• 由来する生物種: Pseudomonas phage Pf1 (Bacteriophage Pf1)
• 細胞内の位置: Virion (Potential): P03621
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 4612.39

構造登録者

Park, S.,Son, W.,Mukhopadhyay, R.,Valafar, H.,Opella, S.J. (登録日: 2009-07-08, 公開日: 2009-10-06, 最終更新日: 2024-05-22)

主引用文献

Park, S.H.,Son, W.S.,Mukhopadhyay, R.,Valafar, H.,Opella, S.J.
Phage-induced alignment of membrane proteins enables the measurement and structural analysis of residual dipolar couplings with dipolar waves and lambda-maps.
J.Am.Chem.Soc., 131:14140-14141, 2009

PubMed Abstract: At pH > 6 added filamentous bacteriophage fd is compatible with many of the detergents used to solubilize membrane proteins for solution NMR studies of membrane proteins and, therefore, serves as an alignment media. In combination with strained polyacrylamide gel alignment, Dipolar Waves can be used to directly assess the secondary structure and a lambda-map extracts the order tensors for de novo structure calculation of membrane proteins without distance restraints.

PubMed: 19761238
DOI: 10.1021/ja905640d

実験手法

SOLUTION NMR