2KLL

Solution structure of human interleukin-33

Summary for 2KLL

• Entry DOI: 10.2210/pdb2kll/pdb
• NMR Information: BMRB: 16317
• Descriptor: Interleukin-33 (1 entity in total)
• Functional Keywords: interleukin, beta-trefoil, cytokine, polymorphism, secreted
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 1
• Total formula weight: 18159.30

Authors

Lingel, A.,Fairbrother, W. (deposition date: 2009-07-06, release date: 2009-11-17, Last modification date: 2024-05-08)

Primary citation

Lingel, A.,Weiss, T.M.,Niebuhr, M.,Pan, B.,Appleton, B.A.,Wiesmann, C.,Bazan, J.F.,Fairbrother, W.J.
Structure of IL-33 and its interaction with the ST2 and IL-1RAcP receptors--insight into heterotrimeric IL-1 signaling complexes.
Structure, 17:1398-1410, 2009

PubMed Abstract: Members of the interleukin-1 (IL-1) family of cytokines play major roles in host defense and immune system regulation in infectious and inflammatory diseases. IL-1 cytokines trigger a biological response in effector cells by assembling a heterotrimeric signaling complex with two IL-1 receptor chains, a high-affinity primary receptor and a low-affinity coreceptor. To gain insights into the signaling mechanism of the novel IL-1-like cytokine IL-33, we first solved its solution structure and then performed a detailed biochemical and structural characterization of the interaction between IL-33, its primary receptor ST2, and the coreceptor IL-1RAcP. Using nuclear magnetic resonance data, we obtained a model of the IL-33/ST2 complex in solution that is validated by small-angle X-ray scattering (SAXS) data and is similar to the IL-1beta/IL-1R1 complex. We extended our SAXS analysis to the IL-33/ST2/IL-1RAcP and IL-1beta/IL-1R1/IL-1RAcP complexes and propose a general model of the molecular architecture of IL-1 ternary signaling complexes.

PubMed: 19836339
DOI: 10.1016/j.str.2009.08.009

Experimental method

SOLUTION NMR