2KLD

Solution Structure of the Calcium Binding Domain of the C-terminal Cytosolic Domain of Polycystin-2

2KLD の概要

• エントリーDOI: 10.2210/pdb2kld/pdb
• 関連するPDBエントリー: 2KLE
• 分子名称: Polycystin-2 (1 entity in total)
• 機能のキーワード: pc2, pkd2, calcium binding domain, ef hand, cytosolic, calcium, coiled coil, disease mutation, glycoprotein, ion transport, ionic channel, membrane, phosphoprotein, polymorphism, transmembrane, transport, membrane protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Membrane; Multi-pass membrane protein (Potential): Q13563
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14024.42

構造登録者

Kalbitzer, H.R. (登録日: 2009-07-01, 公開日: 2009-07-28, 最終更新日: 2024-05-29)

主引用文献

Schumann, F.H.,Hoffmeister, H.,Schmidt, M.,Bader, R.,Besl, E.,Witzgall, R.,Kalbitzer, H.R.
NMR-assignments of a cytosolic domain of the C-terminus of polycystin-2
Biomol.Nmr Assign., 3:141-144, 2009

PubMed Abstract: Mutations in the PKD2 gene lead to the development of polycystic kidney disease (PKD). The PKD2 gene codes for polycystin-2, a cation channel with unknown function. The cytoplasmic, C-terminal domain interacts with a large number of proteins including mDia1, alpha-actinin, PIGEA-14, troponin, and tropomyosin. The C-terminal fragment polycystin-2 (680-796) consisting of 117 amino acids contains a putative calcium binding EF-hand. It was produced in Escherichia coli and enriched uniformly with (13)C and (15)N. The backbone and side chain resonances were assigned by multidimensional NMR methods, the obtained chemical shifts are typical for a partially folded protein. The chemical shifts obtained are in line with the existence of two paired helix-loop-helix (HLH) motifs.

PubMed: 19636966
DOI: 10.1007/s12104-009-9160-x

実験手法

SOLUTION NMR