2KK2
NMR solution structure of the pheromone En-A1 from Euplotes nobilii
Summary for 2KK2
| Entry DOI | 10.2210/pdb2kk2/pdb |
| Related | 2jms 2kc6 2nsv 2nsw |
| NMR Information | BMRB: 16350 |
| Descriptor | En-A1 (1 entity in total) |
| Functional Keywords | signaling protein |
| Biological source | Euplotes nobilii |
| Total number of polymer chains | 1 |
| Total formula weight | 6523.17 |
| Authors | Pedrini, B.,Alimenti, C.,Vallesi, A.,Luporini, P.,Wuthrich, K. (deposition date: 2009-06-15, release date: 2010-05-12, Last modification date: 2024-10-30) |
| Primary citation | Di Giuseppe, G.,Erra, F.,Dini, F.,Alimenti, C.,Vallesi, A.,Pedrini, B.,Wuthrich, K.,Luporini, P. Antarctic and Arctic populations of the ciliate Euplotes nobilii show common pheromone-mediated cell-cell signaling and cross-mating. Proc.Natl.Acad.Sci.USA, 108:3181-3186, 2011 Cited by PubMed Abstract: Wild-type strains of the protozoan ciliate Euplotes collected from different locations on the coasts of Antarctica, Tierra del Fuego and the Arctic were taxonomically identified as the morpho-species Euplotes nobilii, based on morphometric and phylogenetic analyses. Subsequent studies of their sexual interactions revealed that mating combinations of Antarctic and Arctic strains form stable pairs of conjugant cells. These conjugant pairs were isolated and shown to complete mutual gene exchange and cross-fertilization. The biological significance of this finding was further substantiated by demonstrating that close homology exists among the three-dimensional structures determined by NMR of the water-borne signaling pheromones that are constitutively secreted into the extracellular space by these interbreeding strains, in which these molecules trigger the switch between the growth stage and the sexual stage of the life cycle. The fact that Antarctic and Arctic E. nobilii populations share the same gene pool and belong to the same biological species provides new support to the biogeographic model of global distribution of eukaryotic microorganisms, which had so far been based exclusively on studies of morphological and phylogenetic taxonomy. PubMed: 21300903DOI: 10.1073/pnas.1019432108 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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