2KK2

NMR solution structure of the pheromone En-A1 from Euplotes nobilii

2KK2 の概要

• エントリーDOI: 10.2210/pdb2kk2/pdb
• 関連するPDBエントリー: 2jms 2kc6 2nsv 2nsw
• NMR情報: BMRB: 16350
• 分子名称: En-A1 (1 entity in total)
• 機能のキーワード: signaling protein
• 由来する生物種: Euplotes nobilii
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 6523.17

構造登録者

Pedrini, B.,Alimenti, C.,Vallesi, A.,Luporini, P.,Wuthrich, K. (登録日: 2009-06-15, 公開日: 2010-05-12, 最終更新日: 2024-10-30)

主引用文献

Di Giuseppe, G.,Erra, F.,Dini, F.,Alimenti, C.,Vallesi, A.,Pedrini, B.,Wuthrich, K.,Luporini, P.
Antarctic and Arctic populations of the ciliate Euplotes nobilii show common pheromone-mediated cell-cell signaling and cross-mating.
Proc.Natl.Acad.Sci.USA, 108:3181-3186, 2011

PubMed Abstract: Wild-type strains of the protozoan ciliate Euplotes collected from different locations on the coasts of Antarctica, Tierra del Fuego and the Arctic were taxonomically identified as the morpho-species Euplotes nobilii, based on morphometric and phylogenetic analyses. Subsequent studies of their sexual interactions revealed that mating combinations of Antarctic and Arctic strains form stable pairs of conjugant cells. These conjugant pairs were isolated and shown to complete mutual gene exchange and cross-fertilization. The biological significance of this finding was further substantiated by demonstrating that close homology exists among the three-dimensional structures determined by NMR of the water-borne signaling pheromones that are constitutively secreted into the extracellular space by these interbreeding strains, in which these molecules trigger the switch between the growth stage and the sexual stage of the life cycle. The fact that Antarctic and Arctic E. nobilii populations share the same gene pool and belong to the same biological species provides new support to the biogeographic model of global distribution of eukaryotic microorganisms, which had so far been based exclusively on studies of morphological and phylogenetic taxonomy.

PubMed: 21300903
DOI: 10.1073/pnas.1019432108

実験手法

SOLUTION NMR