2KJA

Chemical shift assignments, constraints, and coordinates for CN5 scorpion toxin

2KJA の概要

• エントリーDOI: 10.2210/pdb2kja/pdb
• 分子名称: Beta-toxin Cn5 (1 entity in total)
• 機能のキーワード: nmr solution structure, scorpion toxin, centruroides noxius, centruroides suffusus suffusus, na +channel inhibitor, disulfide bond, ionic channel inhibitor, neurotoxin, secreted, sodium channel inhibitor, toxin
• 由来する生物種: Centruroides noxius (Mexican scorpion)
• 細胞内の位置: Secreted: P45663
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7152.13

構造登録者

Prochnicka-Chalufour, A.,Corzo, G.,Garcia, B.I.,Possani, L.D.,Delepierre, M. (登録日: 2009-05-26, 公開日: 2009-10-06, 最終更新日: 2024-10-30)

主引用文献

Corzo, G.,Prochnicka-Chalufour, A.,Garcia, B.I.,Possani, L.D.,Delepierre, M.
Solution structure of Cn5, a crustacean toxin found in the venom of the scorpions Centruroides noxius and Centruroides suffusus suffusus.
Biochim.Biophys.Acta, 1794:1591-1598, 2009

PubMed Abstract: The crustacean toxin Cn5 from Centruroides noxius Hoffmann and peptide Css39.8 from Centruroides suffusus suffusus scorpion venoms are identical peptides, as confirmed by amino acid sequence of purified toxins and by DNA sequencing of the two respective cloned genes. Therefore in this communication they will be simply named Cn5. Cn5 is a 66 amino acid long peptide with four disulfide bridges, formed between pairs of cysteines: C1-C8, C2-C5, C3-C6, and C4-C7 (the numbers indicate the relative positions of the cysteine residues in the primary structure). This peptide is non-toxic to mammals but deadly to arthropods (LD(50) 28.5 mg/g body weight of crayfish). Its three-dimensional structure was determined by NMR using a total of 965 meaningful distance constraints derived from the volume integration of the 2D NOESY spectra. The Cn5 structure displays a mixed alpha/beta fold stabilized by four disulfide bridges, with a kink induced by a cis-proline in its C-terminal part. Cn5 electrostatic surface is compared to that of Cn2 toxin toxic to mammals. The local differences produced by additional or substituted residues that would influence toxin selectivity towards mammalian or crustacean Na(+) channels are discussed.

PubMed: 19631296
DOI: 10.1016/j.bbapap.2009.07.006

実験手法

SOLUTION NMR