2KIX

Channel domain of BM2 protein from influenza B virus

2KIX の概要

• エントリーDOI: 10.2210/pdb2kix/pdb
• 分子名称: BM2 protein (1 entity in total)
• 機能のキーワード: bm2, influenza b, channel, transport protein
• 由来する生物種: Influenza B virus (B/Taiwan/70061/2006) (B/Taiwan/70061/2006)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 15318.23

構造登録者

Wang, J.,Pielak, R.,McClintock, M.,Chou, J. (登録日: 2009-05-12, 公開日: 2009-11-10, 最終更新日: 2024-05-22)

主引用文献

Wang, J.,Pielak, R.M.,McClintock, M.A.,Chou, J.J.
Solution structure and functional analysis of the influenza B proton channel.
Nat.Struct.Mol.Biol., 16:1267-1271, 2009

PubMed Abstract: Influenza B virus contains an integral membrane protein, BM2, that oligomerizes in the viral membrane to form a pH-activated proton channel. Here we report the solution structures of both the membrane-embedded channel domain and the cytoplasmic domain of BM2. The channel domain assumes a left-handed coiled-coil tetramer formation with a helical packing angle of -37 degrees to form a polar pore in the membrane for conducting ions. Mutagenesis and proton flux experiments identified residues involved in proton relay and suggest a mechanism of proton conductance. The cytoplasmic domain of BM2 also forms a coiled-coil tetramer. It has a bipolar charge distribution, in which a negatively charged region interacts specifically with the M1 matrix protein that is involved in packaging the genome in the virion. This interaction suggests BM2 also recruits matrix proteins to the cell surface during virus budding, making BM2 an unusual membrane protein with the dual roles of conducting ions and recruiting proteins to the membrane.

PubMed: 19898475
DOI: 10.1038/nsmb.1707

実験手法

SOLUTION NMR