2KI2

Solution Structure of ss-DNA Binding Protein 12RNP2 Precursor, HP0827(O25501_HELPY) form Helicobacter pylori

2KI2 の概要

• エントリーDOI: 10.2210/pdb2ki2/pdb
• 分子名称: Ss-DNA binding protein 12RNP2 (1 entity in total)
• 機能のキーワード: hp0827, helicobacter pylori, rrm, rnp1, rnp2, ss-dna binding proteins, rna binding protein-ss-dna binding protein complex, rna binding protein/ss-dna binding protein
• 由来する生物種: Helicobacter pylori (Campylobacter pylori)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 10469.88

構造登録者

Ma, C.,Lee, J.,Kim, J.,Park, S.,Kwon, A.,Lee, B. (登録日: 2009-04-20, 公開日: 2009-10-20, 最終更新日: 2024-05-29)

主引用文献

Jang, S.B.,Ma, C.,Lee, J.Y.,Kim, J.H.,Park, S.J.,Kwon, A.R.,Lee, B.J.
NMR solution structure of HP0827 (O25501_HELPY) from Helicobacter pylori: model of the possible RNA-binding site
J.Biochem., 146:667-674, 2009

PubMed Abstract: The HP0827 protein is an 82-residue protein identified as a putative ss-DNA-binding protein 12RNP2 Precursor from Helicobacter pylori. Here, we have determined 3D structure of HP0827 using Nuclear Magnetic Resonance. It has a ferredoxin-like fold, beta1-alpha1-beta2-beta3-alpha2-beta4 (alpha; alpha-helix and beta; beta-sheet) and ribonucleoprotein (RNP) motifs which are thought to be important in RNA binding. By using structural homologues search and analyzing electrostatic potential of surface, we could compared HP0827 with other RNA-binding proteins (sex-lethal, T-cell restricted intracellular antigen-1, U1A) to predict RNA-binding sites of HP0827. We could predict that beta sheets of HP0827, especially beta1 and beta3, are primary region for RNA binding. Consequently, similar to other RNA-binding proteins, RNP motifs (Y5, F45, F47), positively charged and hydrophobic regions (K32, R37, K40, K41, K43, R70, R73) are proposed as a putative RNA-binding sites. In addition, differences in amino acids composition of RNP motifs, N, C-terminal residues, loop-region fold and the orientation of alpha1-helix with other RNA recognition motif proteins could give specific biological functions to HP0827. Finally, the study on natural RNA target is also important to completely understand the biological function of HP0827.

PubMed: 19605462
DOI: 10.1093/jb/mvp105

実験手法

SOLUTION NMR