2KHY

Specifier Domain of B. subtilis tyrS T box leader RNA

2KHY の概要

• エントリーDOI: 10.2210/pdb2khy/pdb
• 分子名称: RNA (38-MER) (1 entity in total)
• 機能のキーワード: specifier domain, bacillus, s-turn, loop-e, rna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12224.33

構造登録者

Wang, J.,Henkin, T.M. (登録日: 2009-04-14, 公開日: 2010-02-16, 最終更新日: 2024-05-22)

主引用文献

Wang, J.,Henkin, T.M.,Nikonowicz, E.P.
NMR structure and dynamics of the Specifier Loop domain from the Bacillus subtilis tyrS T box leader RNA.
Nucleic Acids Res., 38:3388-3398, 2010

PubMed Abstract: Gram-positive bacteria utilize a tRNA-responsive transcription antitermination mechanism, designated the T box system, to regulate expression of many amino acid biosynthetic and aminoacyl-tRNA synthetase genes. The RNA transcripts of genes controlled by this mechanism contain 5' untranslated regions, or leader RNAs, that specifically bind cognate tRNA molecules through pairing of nucleotides in the tRNA anticodon loop with nucleotides in the Specifier Loop domain of the leader RNA. We have determined the solution structure of the Specifier Loop domain of the tyrS leader RNA from Bacillus subtilis. Fifty percent of the nucleotides in the Specifier Loop domain adopt a loop E motif. The Specifier Sequence nucleotides, which pair with the tRNA anticodon, stack with their Watson-Crick edges rotated toward the minor groove and exhibit only modest flexibility. We also show that a Specifier Loop domain mutation that impairs the function of the B. subtilis glyQS T box RNA disrupts the tyrS loop E motif. Our results suggest a mechanism for tRNA-Specifier Loop binding in which the phosphate backbone kink created by the loop E motif causes the Specifier Sequence bases to rotate toward the minor groove, which increases accessibility for pairing with bases in the anticodon loop of tRNA.

PubMed: 20110252
DOI: 10.1093/nar/gkq020

実験手法

SOLUTION NMR