2KHC

Bruno RRM3+

Summary for 2KHC

• Entry DOI: 10.2210/pdb2khc/pdb
• NMR Information: BMRB: 16236
• Descriptor: Testis-specific RNP-type RNA binding protein (1 entity in total)
• Functional Keywords: rrm, rna recognition motif, bruno, rna binding protein
• Biological source: Drosophila melanogaster (Fruit fly)
• Total number of polymer chains: 1
• Total formula weight: 12296.02

Authors

Lyon, A.M.,Reveal, B.S.,Macdonald, P.M.,Hoffman, D.W. (deposition date: 2009-04-01, release date: 2009-12-22, Last modification date: 2024-05-22)

Primary citation

Lyon, A.M.,Reveal, B.S.,Macdonald, P.M.,Hoffman, D.W.
Bruno protein contains an expanded RNA recognition motif.
Biochemistry, 48:12202-12212, 2009

PubMed Abstract: The RNA recognition motif (or RRM) is a ubiquitous RNA-binding module present in approximately 2% of the proteins encoded in the human genome. This work characterizes an expanded RRM, which is present in the Drosophila Bruno protein, and targets regulatory elements in the oskar mRNA through which Bruno controls translation. In this Bruno RRM, the deletion of 40 amino acids prior to the N-terminus of the canonical RRM resulted in a significantly decreased affinity of the protein for its RNA target. NMR spectroscopy showed that the expanded Bruno RRM contains the familiar RRM fold of four antiparallel beta-strands and two alpha-helices, preceded by a 10-residue loop that contacts helix alpha(1) and strand beta(2); additional amino acids at the N-terminus of the domain are relatively flexible in solution. NMR results also showed that a truncated form of the Bruno RRM, lacking the flexible N-terminal amino acids, forms a stable and complete canonical RRM, so that the loss of RNA binding activity cannot be attributed to disruption of the RRM fold. This expanded Bruno RRM provides a new example of the features that are important for RNA recognition by an RRM-containing protein.

PubMed: 19919093
DOI: 10.1021/bi900624j

Experimental method

SOLUTION NMR