2KH9

Solution structure of yeast Prp24-RRM2 bound to a fragment of U6 RNA

Summary for 2KH9

• Entry DOI: 10.2210/pdb2kh9/pdb
• NMR Information: BMRB: 16230,16243,16244,16246
• Descriptor: U4/U6 snRNA-associated-splicing factor PRP24, 5'-R(*AP*GP*AP*GP*AP*U)-3' (2 entities in total)
• Functional Keywords: protein/rna, rrm, snrnp, splicing, mrna processing, mrna splicing, nucleus, phosphoprotein, rna-binding, splicing-rna complex, splicing/rna
• Biological source: Saccharomyces cerevisiae (yeast); More
• Cellular location: Nucleus: P49960
• Total number of polymer chains: 2
• Total formula weight: 12780.63

Authors

Martin-Tumasz, S.A.,Butcher, S.E. (deposition date: 2009-03-27, release date: 2010-03-09, Last modification date: 2024-05-22)

Primary citation

Martin-Tumasz, S.,Reiter, N.J.,Brow, D.A.,Butcher, S.E.
Structure and functional implications of a complex containing a segment of U6 RNA bound by a domain of Prp24.
Rna, 16:792-804, 2010

PubMed Abstract: U6 RNA plays a critical role in pre-mRNA splicing. Assembly of U6 into the spliceosome requires a significant structural rearrangement and base-pairing with U4 RNA. In the yeast Saccharomyces cerevisiae, this process requires the essential splicing factor Prp24. We present the characterization and structure of a complex containing one of Prp24's four RNA recognition motif (RRM) domains, RRM2, and a fragment of U6 RNA. NMR methods were used to identify the preferred U6 binding sequence of RRM2 (5'-GAGA-3'), measure the affinity of the interaction, and solve the structure of RRM2 bound to the hexaribonucleotide AGAGAU. Interdomain contacts observed between RRM2 and RRM3 in a crystal structure of the free protein are not detectable in solution. A structural model of RRM1 and RRM2 bound to a longer segment of U6 RNA is presented, and a partial mechanism for Prp24's annealing activity is proposed.

PubMed: 20181740
DOI: 10.1261/rna.1913310

Experimental method

SOLUTION NMR