2KFK

Solution structure of Bem1p PB1 domain complexed with Cdc24p PB1 domain

2KFK の概要

• エントリーDOI: 10.2210/pdb2kfk/pdb
• 分子名称: Bud emergence protein 1, Cell division control protein 24 (2 entities in total)
• 機能のキーワード: pb1, budding, yeast, phox, signaling protein
• 由来する生物種: Saccharomyces cerevisiae (yeast); 詳細
• 細胞内の位置: Cytoplasm, cytoskeleton: P29366
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 19030.78

構造登録者

Kobashigawa, Y.,Yoshinaga, S.,Tandai, T.,Ogura, K.,Inagaki, F. (登録日: 2009-02-23, 公開日: 2009-10-06, 最終更新日: 2024-05-29)

主引用文献

Ogura, K.,Tandai, T.,Yoshinaga, S.,Kobashigawa, Y.,Kumeta, H.,Ito, T.,Sumimoto, H.,Inagaki, F.
NMR structure of the heterodimer of Bem1 and Cdc24 PB1 domains from Saccharomyces cerevisiae
J.Biochem., 146:317-325, 2009

PubMed Abstract: Bem1 and Cdc24 of the budding yeast Saccharomyces cerevisiae interact with each other through PB1-PB1 heterodimer formation to regulate the establishment of cell polarity. Here we present the tertiary structure of the heterodimer of Bem1 and Cdc24 PB1 domains determined by NMR spectroscopy. To avoid ambiguity in the NMR spectral analysis, we first prepared a mutant of the Cdc24 PB1 domain that had truncated loops. The mutant provided well dispersed spectra without spectral overlapping, thus allowing unambiguous spectral assignments for structure determination. We confirmed that the loop deletion-mutant was quite similar to the wild-type in both 3D structure and binding affinity. The NMR structure of the heterodimer of the deletion-mutant of Cdc24 PB1 and Bem1 PB1 was determined using a variety of isotope labelled samples including perdeuteration. The interface between the Bem1/Cdc24 PB1 heterodimer was analysed at atomic resolution. Through a comparison with the tertiary structures of other PB1-PB1 heterodimers, we found that conserved electrostatic properties on the molecular surface were commonly used for PB1-PB1 interaction, but hydrophobic interactions were important for cognate interaction in Bem1/Cdc24 PB1 heterodimer formation.

PubMed: 19451149
DOI: 10.1093/jb/mvp075

実験手法

SOLUTION NMR