2KEQ
Solution structure of DnaE intein from Nostoc punctiforme
Summary for 2KEQ
| Entry DOI | 10.2210/pdb2keq/pdb |
| Descriptor | DNA polymerase III alpha subunit, Nucleic acid binding OB-fold tRNA/helicase-type (1 entity in total) |
| Functional Keywords | intein, dnae intein, protein splicing, protein trans splicing, splicing |
| Biological source | Nostoc punctiforme PCC 73102 |
| Total number of polymer chains | 1 |
| Total formula weight | 15932.98 |
| Authors | Oeemig, J.S.,Aranko, A.S.,Djupsj, J.B.,Iwai, H. (deposition date: 2009-02-02, release date: 2009-05-19, Last modification date: 2024-05-29) |
| Primary citation | Oeemig, J.S.,Aranko, A.S.,Djupsjobacka, J.,Heinamaki, K.,Iwai, H. Solution structure of DnaE intein from Nostoc punctiforme: structural basis for the design of a new split intein suitable for site-specific chemical modification. Febs Lett., 583:1451-1456, 2009 Cited by PubMed Abstract: Naturally split DnaE intein from Nostoc punctiforme (Npu) has robust protein trans-splicing activity and high tolerance of sequence variations at the splicing junctions. We determined the solution structure of a single chain variant of NpuDnaE intein by NMR spectroscopy. Based on the NMR structure and the backbone dynamics of the single chain NpuDnaE intein, we designed a functional split variant of the NpuDnaE intein having a short C-terminal half (C-intein) composed of six residues. In vivo and in vitro protein ligation of model proteins by the newly designed split intein were demonstrated. PubMed: 19344715DOI: 10.1016/j.febslet.2009.03.058 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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