2KEL

Structure of the transcription regulator SvtR from the hyperthermophilic archaeal virus SIRV1

2KEL の概要

• エントリーDOI: 10.2210/pdb2kel/pdb
• NMR情報: BMRB: 16153
• 分子名称: Uncharacterized protein 56B (1 entity in total)
• 機能のキーワード: protein, homodimer, ribbon-helix-helix, transcription repressor
• 由来する生物種: Sulfolobus islandicus rod-shaped virus 1 (SIRV-1)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 13265.45

構造登録者

Guilliere, F.,Kessler, A.,Peixeiro, N.,Sezonov, G.,Prangishvili, D.,Delepierre, M.,Guijarro, J.I. (登録日: 2009-01-30, 公開日: 2009-06-16, 最終更新日: 2024-05-22)

主引用文献

Guilliere, F.,Peixeiro, N.,Kessler, A.,Raynal, B.,Desnoues, N.,Keller, J.,Delepierre, M.,Prangishvili, D.,Sezonov, G.,Guijarro, J.I.
Structure, function, and targets of the transcriptional regulator SvtR from the hyperthermophilic archaeal virus SIRV1.
J.Biol.Chem., 284:22222-22237, 2009

PubMed Abstract: We have characterized the structure and the function of the 6.6-kDa protein SvtR (formerly called gp08) from the rod-shaped virus SIRV1, which infects the hyperthermophilic archaeon Sulfolobus islandicus that thrives at 85 degrees C in hot acidic springs. The protein forms a dimer in solution. The NMR solution structure of the protein consists of a ribbon-helix-helix (RHH) fold between residues 13 and 56 and a disordered N-terminal region (residues 1-12). The structure is very similar to that of bacterial RHH proteins despite the low sequence similarity. We demonstrated that the protein binds DNA and uses its beta-sheet face for the interaction like bacterial RHH proteins. To detect all the binding sites on the 32.3-kb SIRV1 linear genome, we designed and performed a global genome-wide search of targets based on a simplified electrophoretic mobility shift assay. Four targets were recognized by the protein. The strongest binding was observed with the promoter of the gene coding for a virion structural protein. When assayed in a host reconstituted in vitro transcription system, the protein SvtR (Sulfolobus virus transcription regulator) repressed transcription from the latter promoter, as well as from the promoter of its own gene.

PubMed: 19535331
DOI: 10.1074/jbc.M109.029850

実験手法

SOLUTION NMR