2KEB

NMR solution structure of the N-terminal domain of the DNA polymerase alpha p68 subunit

2KEB の概要

• エントリーDOI: 10.2210/pdb2keb/pdb
• 分子名称: DNA polymerase subunit alpha B (1 entity in total)
• 機能のキーワード: dna polymerase alpha, dna replication, nucleus, phosphoprotein, dna binding protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus: Q14181
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 11151.53

構造登録者

Huang, H.,Weiner, B.E.,Zhang, H.,Fuller, B.E.,Gao, Y.,Wile, B.M.,Chazin, W.J.,Fanning, E. (登録日: 2009-01-28, 公開日: 2010-02-02, 最終更新日: 2024-05-22)

主引用文献

Huang, H.,Weiner, B.E.,Zhang, H.,Fuller, B.E.,Gao, Y.,Wile, B.M.,Zhao, K.,Arnett, D.R.,Chazin, W.J.,Fanning, E.
Structure of a DNA polymerase alpha-primase domain that docks on the SV40 helicase and activates the viral primosome.
J.Biol.Chem., 285:17112-17122, 2010

PubMed Abstract: DNA polymerase alpha-primase (pol-prim) plays a central role in DNA replication in higher eukaryotes, initiating synthesis on both leading and lagging strand single-stranded DNA templates. Pol-prim consists of a primase heterodimer that synthesizes RNA primers, a DNA polymerase that extends them, and a fourth subunit, p68 (also termed B-subunit), that is thought to regulate the complex. Although significant knowledge about single-subunit primases of prokaryotes has accumulated, the functions and regulation of pol-prim remain poorly understood. In the SV40 replication model, the p68 subunit is required for primosome activity and binds directly to the hexameric viral helicase T antigen, suggesting a functional link between T antigen-p68 interaction and primosome activity. To explore this link, we first mapped the interacting regions of the two proteins and discovered a previously unrecognized N-terminal globular domain of p68 (p68N) that physically interacts with the T antigen helicase domain. NMR spectroscopy was used to determine the solution structure of p68N and map its interface with the T antigen helicase domain. Structure-guided mutagenesis of p68 residues in the interface diminished T antigen-p68 interaction, confirming the interaction site. SV40 primosome activity of corresponding pol-prim mutants decreased in proportion to the reduction in p68N-T antigen affinity, confirming that p68-T antigen interaction is vital for primosome function. A model is presented for how this interaction regulates SV40 primosome activity, and the implications of our findings are discussed in regard to the molecular mechanisms of eukaryotic DNA replication initiation.

PubMed: 20234039
DOI: 10.1074/jbc.M110.116830

実験手法

SOLUTION NMR