2KCF

The NMR solution structure of the isolated Apo Pin1 WW domain

2KCF の概要

• エントリーDOI: 10.2210/pdb2kcf/pdb
• 関連するPDBエントリー: 2kbu
• 分子名称: Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (1 entity in total)
• 機能のキーワード: isomerase, peptidylprolyl isomerase, pin1, ww domain, cell cycle, nucleus, phosphoprotein, rotamase
• 由来する生物種: Homo sapiens
• 細胞内の位置: Nucleus: Q13526
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 4174.64

構造登録者

Kowalski, J.A.,Liu, K.,Kelly, J.W. (登録日: 2008-12-19, 公開日: 2009-01-13, 最終更新日: 2024-05-22)

主引用文献

Kowalski, J.A.,Liu, K.,Kelly, J.W.
NMR solution structure of the isolated Apo Pin1 WW domain: comparison to the x-ray crystal structures of Pin1
Biopolymers, 63:111-121, 2002

PubMed Abstract: The NMR solution structure of the isolated Apo Pin1 WW domain (6-39) reveals that it adopts a twisted three-stranded antiparallel beta-sheet conformation, very similar to the structure exhibited by the crystal of this domain in the context of the two domain Pin1 protein. While the B factors in the apo x-ray crystal structure indicate that loop 1 and loop 2 are conformationally well defined, the solution NMR data suggest that loop 1 is quite flexible, at least in the absence of the ligand. The NMR chemical shift and nuclear Overhauser effect pattern exhibited by the 6-39 Pin1 WW domain has proven to be diagnostic for demonstrating that single site variants of this domain adopt a normally folded structure. Knowledge of this type is critical before embarking on time-consuming kinetic and thermodynamic studies required for a detailed understanding of beta-sheet folding.

PubMed: 11786999

実験手法

SOLUTION NMR