2KC6

NMR solution structure of the pheromone En-1 of Euplotes nobilii at -1.5 C

2KC6 の概要

• エントリーDOI: 10.2210/pdb2kc6/pdb
• 関連するPDBエントリー: 2nsv
• NMR情報: BMRB: 16062
• 分子名称: Mating pheromone En-1 (1 entity in total)
• 機能のキーワード: pheromone, secreted, signaling protein
• 由来する生物種: Euplotes nobilii
• 細胞内の位置: Secreted: P83441
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 5630.08

構造登録者

Pedrini, B.,Alimenti, C.,Vallesi, A.,Luporini, P.,Wuthrich, K. (登録日: 2008-12-17, 公開日: 2009-08-04, 最終更新日: 2024-10-16)

主引用文献

Alimenti, C.,Vallesi, A.,Pedrini, B.,Wuthrich, K.,Luporini, P.
Molecular cold-adaptation: Comparative analysis of two homologous families of psychrophilic and mesophilic signal proteins of the protozoan ciliate, Euplotes.
Iubmb Life, 61:838-845, 2009

PubMed Abstract: Unique opportunities are provided by phylogenetically closely related organisms thriving in stably cold, or temperate milieus to study adaptive modifications of structurally homologous molecules. These modifications are of keen interest in basic science as well as in biotechnology. This review highlights structural and functional specificities that differentiate two homologous families of psychrophilic and mesophilic water-borne proteins (designated as pheromones) that signal mitotic growth and sexual mating in two marine species of the protozoan ciliate Euplotes, i.e., E. nobilii, which is distributed in Antarctic and Arctic waters, and E. raikovi, which inhabits temperate waters. The two protein families show strict conservation of a common three-helix bundle in a compact core of the molecular structure, which provides long-lasting integrity and biological activity to these molecules in their natural environment. In the psychrophilic pheromone family, cold-adaptation appears to have been achieved by superimposing an integrated complex of structural modifications on this conserved scaffold. Functionally most relevant appear to be extensions of polypeptide segments devoid of regular secondary structures, a specific distribution of polar and hydrophobic amino acids, the presence of solvent-exposed clusters of negatively charged amino acid side chains, and a unique role of aromatic residues in anchoring the molecular architecture. Due to these modifications, the psychrophilic pheromones are an example of an elegant combination of high stability of the three-dimensional structures with sufficient structural plasticity for efficient functioning at their physiologically low temperatures.

PubMed: 19621350
DOI: 10.1002/iub.228

実験手法

SOLUTION NMR