2KC2

NMR structure of the F1 domain (residues 86-202) of the talin

2KC2 の概要

• エントリーDOI: 10.2210/pdb2kc2/pdb
• 関連するPDBエントリー: 2kC1
• 分子名称: Talin-1 (1 entity in total)
• 機能のキーワード: talin, ferm, f1, adhesion, cell membrane, cell projection, cytoplasm, cytoskeleton, membrane, phosphoprotein, structural protein
• 由来する生物種: Mus musculus (mouse)
• 細胞内の位置: Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side (By similarity): P26039
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15030.34

構造登録者

Goult, B.T.,Elliott, P.R.,Roberts, G.C.K.,Critchely, D.R.,Barsukov, I.L. (登録日: 2008-12-13, 公開日: 2010-01-19, 最終更新日: 2024-05-29)

主引用文献

Goult, B.T.,Bouaouina, M.,Elliott, P.R.,Bate, N.,Patel, B.,Gingras, A.R.,Grossmann, J.G.,Roberts, G.C.,Calderwood, D.A.,Critchley, D.R.,Barsukov, I.L.
Structure of a double ubiquitin-like domain in the talin head: a role in integrin activation.
Embo J., 29:1069-1080, 2010

PubMed Abstract: Talin is a 270-kDa protein that activates integrins and couples them to cytoskeletal actin. Talin contains an N-terminal FERM domain comprised of F1, F2 and F3 domains, but it is atypical in that F1 contains a large insert and is preceded by an extra domain F0. Although F3 contains the binding site for beta-integrin tails, F0 and F1 are also required for activation of beta1-integrins. Here, we report the solution structures of F0, F1 and of the F0F1 double domain. Both F0 and F1 have ubiquitin-like folds joined in a novel fixed orientation by an extensive charged interface. The F1 insert forms a loop with helical propensity, and basic residues predicted to reside on one surface of the helix are required for binding to acidic phospholipids and for talin-mediated activation of beta1-integrins. This and the fact that basic residues on F2 and F3 are also essential for integrin activation suggest that extensive interactions between the talin FERM domain and acidic membrane phospholipids are required to orientate the FERM domain such that it can activate integrins.

PubMed: 20150896
DOI: 10.1038/emboj.2010.4

実験手法

SOLUTION NMR