2KBP

Solution structure of a G-quadruplex of human telomeric RNA

Summary for 2KBP

• Entry DOI: 10.2210/pdb2kbp/pdb
• Descriptor: 5'-R(*UP*AP*GP*GP*GP*UP*UP*AP*GP*GP*GP*U)-3' (1 entity in total)
• Functional Keywords: g-quadruplex, parallel form, rna, telomeric
• Total number of polymer chains: 2
• Total formula weight: 7818.70

Authors

Phan, A.T.,Martadinata, H. (deposition date: 2008-12-05, release date: 2009-03-24, Last modification date: 2024-05-29)

Primary citation

Martadinata, H.,Phan, A.T.
Structure of propeller-type parallel-stranded RNA G-quadruplexes, formed by human telomeric RNA sequences in K+ solution.
J.Am.Chem.Soc., 131:2570-2578, 2009

PubMed Abstract: Very recent studies showed that mammalian telomeres were transcribed into telomeric-repeat-containing RNAs and suggested that these RNA molecules were biologically important. Here we report on a structural study of RNA G-quadruplexes formed by human telomeric RNA sequences in K(+) solution. Our data indicated that these sequences formed propeller-type parallel-stranded RNA G-quadruplexes. We have determined the NMR-based solution structure of a dimeric propeller-type RNA G-quadruplex formed by the 12-nt human telomeric RNA sequence r(UAGGGUUAGGGU). We also observed the stacking of two such propeller-type G-quadruplex blocks for the 10-nt human telomeric RNA sequence r(GGGUUAGGGU) and a higher-order G-quadruplex structure for the 9-nt human telomeric RNA sequence r(GGGUUAGGG). Based on these findings we proposed how higher-order structures might be formed by long telomeric RNA.

PubMed: 19183046
DOI: 10.1021/ja806592z

Experimental method

SOLUTION NMR