2KAK

Solution structure of the beta-E-domain of wheat Ec-1 metallothionein

2KAK の概要

• エントリーDOI: 10.2210/pdb2kak/pdb
• NMR情報: BMRB: 16025
• 分子名称: EC protein I/II, ZINC ION (2 entities in total)
• 機能のキーワード: metallothionein, solution structure, wheat ec-1, zn binding, metal-binding, metal-thiolate cluster, zinc, metal binding protein
• 由来する生物種: Triticum aestivum (Canadian hard winter wheat,common wheat,wheat)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 5346.25

構造登録者

Peroza, E.A.,Schmucki, R.,Guntert, P.,Freisinger, E.,Zerbe, O. (登録日: 2008-11-06, 公開日: 2009-05-05, 最終更新日: 2024-05-01)

主引用文献

Peroza, E.A.,Schmucki, R.,Guntert, P.,Freisinger, E.,Zerbe, O.
The beta(E)-domain of wheat E(c)-1 metallothionein: a metal-binding domain with a distinctive structure.
J.Mol.Biol., 387:207-218, 2009

PubMed Abstract: Metallothioneins (MTs) are ubiquitous cysteine-rich proteins with a high affinity for divalent metal ions such as Zn(II), Cu(I), and Cd(II) that are involved in metal ion homeostasis and detoxification, as well as protection against reactive oxygen species. Here we show the NMR solution structure of the beta(E)-domain of the early cysteine-labeled protein (E(c)-1) from wheat (beta(E)-E(c)-1), which represents the first three-dimensional structure of a plant MT. The beta(E)-domain comprises the 51 C-terminal residues of E(c)-1 and exhibits a distinctive unprecedented structure with two separate metal-binding centers, a mononuclear Zn(II) binding site constituted by two cysteine and two highly conserved histidine residues as found in certain zinc-finger motifs, and a cluster formed by three Zn(II) ions coordinated by nine Cys residues that resembles the cluster in the beta-domain of vertebrate MTs. Cys-metal ion connectivities were determined by exhaustive structure calculations for all 7560 possible configurations of the three-metal cluster. Backbone dynamics investigated by (15)N relaxation experiments support the results of the structure determination in that beta(E)-E(c)-1 is a rigidly folded polypeptide. To further investigate the influence of metal ion binding on the stability of the structure, we replaced Zn(II) with Cd(II) ions and examined the effects of metal ion release on incubation with a metal ion chelator.

PubMed: 19361445
DOI: 10.1016/j.jmb.2009.01.035

実験手法

SOLUTION NMR