2K9S

Solution structure of dna binding domain of E. coli arac

2K9S の概要

• エントリーDOI: 10.2210/pdb2k9s/pdb
• 関連するPDBエントリー: 1BL0 1D5Y 1XJA 2AAC 2ARA 2ARC
• NMR情報: BMRB: 16001
• 分子名称: Arabinose operon regulatory protein (1 entity in total)
• 機能のキーワード: activator, arabinose catabolism, carbohydrate metabolism, cytoplasm, dna-binding, repressor, transcription, transcription regulation
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12096.71

構造登録者

Rodgers, M.E.,Schleif, R.F. (登録日: 2008-10-23, 公開日: 2008-11-25, 最終更新日: 2024-05-08)

主引用文献

Rodgers, M.E.,Schleif, R.
Solution structure of the DNA binding domain of AraC protein.
Proteins, 77:202-208, 2009

PubMed Abstract: We report the solution structure of the DNA binding domain of the Escherichia coli regulatory protein AraC determined in the absence of DNA. The 20 lowest energy structures, determined on the basis of 1507 unambiguous nuclear Overhauser restraints and 180 angle restraints, are well resolved with a pair wise backbone root mean square deviation of 0.7 A. The protein, free of DNA, is well folded in solution and contains seven helices arranged in two semi-independent sub domains, each containing one helix-turn-helix DNA binding motif, joined by a 19 residue central helix. This solution structure is discussed in the context of extensive biochemical and physiological data on AraC and with respect to the DNA-bound structures of the MarA and Rob homologs.

PubMed: 19422057
DOI: 10.1002/prot.22431

実験手法

SOLUTION NMR