2K9M

Structure of the Core Binding Domain of sigma54

2K9M の概要

• エントリーDOI: 10.2210/pdb2k9m/pdb
• 関連するPDBエントリー: 2K9L
• 分子名称: RNA polymerase sigma factor RpoN (1 entity in total)
• 機能のキーワード: core binding domain, transcription
• 由来する生物種: Aquifex aeolicus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15222.61

構造登録者

Hong, E.,Wemmer, D. (登録日: 2008-10-19, 公開日: 2009-05-26, 最終更新日: 2024-05-22)

主引用文献

Hong, E.,Doucleff, M.,Wemmer, D.E.
Structure of the RNA polymerase core-binding domain of sigma(54) reveals a likely conformational fracture point
J.Mol.Biol., 390:70-82, 2009

PubMed Abstract: Transcription initiation by bacterial sigma(54)-RNA polymerase requires a conformational change of the holopolymerase-DNA complex, driven by an enhancer-binding protein. Although structures of the core polymerase and the more common sigma(70) factor have been determined, little is known about the structure of the sigma(54) variant. We report here the structure of an Aquifex aeolicus sigma(54) domain (residues 69-198), which binds core RNA polymerase. The structure is composed of two distinct subdomains held together by a small, conserved hydrophobic interface that appears to act as a fracture point in the structure. The N-terminal, four-helical subdomain has a negative surface and conserved residues that likely contact the core polymerase, while the C-terminal, three-helical bundle has a strongly positive patch that could contact DNA. Sequence conservation indicates that these structural features are conserved and are important for the role of sigma(54) in the polymerase complex.

PubMed: 19426742
DOI: 10.1016/j.jmb.2009.04.070

実験手法

SOLUTION NMR