2K9D

Solution structure of the domain X of measle phosphoprotein

Summary for 2K9D

• Entry DOI: 10.2210/pdb2k9d/pdb
• Descriptor: Phosphoprotein (1 entity in total)
• Functional Keywords: measle, morbillivirus, phosphoprotein, x domain, rna editing, rna replication, viral protein
• Biological source: Measles virus
• Total number of polymer chains: 1
• Total formula weight: 5169.16

Authors

Gely, S.,Bernard, C.,Bourhis, J.M.,Longhi, S.,Darbon, H. (deposition date: 2008-10-08, release date: 2009-10-20, Last modification date: 2024-05-29)

Primary citation

Bernard, C.,Gely, S.,Bourhis, J.M.,Morelli, X.,Longhi, S.,Darbon, H.
Interaction between the C-terminal domains of N and P proteins of measles virus investigated by NMR.
Febs Lett., 583:1084-1089, 2009

PubMed Abstract: In this paper we investigate the interaction between the C-terminal domains of the measles virus phosphoprotein (XD) and nucleoprotein (N(TAIL)) by using nuclear magnetic resonance chemical shift perturbation experiments. Using both N(TAIL) constructs and peptides, we show that contrary to the conserved Box2 region (N(489-506)), the C-terminal region of N(TAIL) (N(513-525)) does not directly interact with XD, and yet affects binding to XD. We tentatively propose a model where the C-terminus of N(TAIL) would stabilize the N(TAIL)-XD complex either via a functional coupling with N(489-506) or by reducing the entropic penalty associated to the binding-coupled-to-folding process.

PubMed: 19275899
DOI: 10.1016/j.febslet.2009.03.004

Experimental method

SOLUTION NMR