2K9D

Solution structure of the domain X of measle phosphoprotein

2K9D の概要

• エントリーDOI: 10.2210/pdb2k9d/pdb
• 分子名称: Phosphoprotein (1 entity in total)
• 機能のキーワード: measle, morbillivirus, phosphoprotein, x domain, rna editing, rna replication, viral protein
• 由来する生物種: Measles virus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 5169.16

構造登録者

Gely, S.,Bernard, C.,Bourhis, J.M.,Longhi, S.,Darbon, H. (登録日: 2008-10-08, 公開日: 2009-10-20, 最終更新日: 2024-05-29)

主引用文献

Bernard, C.,Gely, S.,Bourhis, J.M.,Morelli, X.,Longhi, S.,Darbon, H.
Interaction between the C-terminal domains of N and P proteins of measles virus investigated by NMR.
Febs Lett., 583:1084-1089, 2009

PubMed Abstract: In this paper we investigate the interaction between the C-terminal domains of the measles virus phosphoprotein (XD) and nucleoprotein (N(TAIL)) by using nuclear magnetic resonance chemical shift perturbation experiments. Using both N(TAIL) constructs and peptides, we show that contrary to the conserved Box2 region (N(489-506)), the C-terminal region of N(TAIL) (N(513-525)) does not directly interact with XD, and yet affects binding to XD. We tentatively propose a model where the C-terminus of N(TAIL) would stabilize the N(TAIL)-XD complex either via a functional coupling with N(489-506) or by reducing the entropic penalty associated to the binding-coupled-to-folding process.

PubMed: 19275899
DOI: 10.1016/j.febslet.2009.03.004

実験手法

SOLUTION NMR