2K9A

The Solution Structure of the Arl2 Effector, BART

2K9A の概要

• エントリーDOI: 10.2210/pdb2k9a/pdb
• 分子名称: ADP-ribosylation factor-like protein 2-binding protein (1 entity in total)
• 機能のキーワード: protein, effector, small g protein, alternative splicing, cytoplasm, mitochondrion, phosphoprotein, protein binding
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm: Q9Y2Y0
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16162.99

構造登録者

Bailey, L.K.,Campbell, L.J.,Evetts, K.A.,Littlefield, K.,Rajendra, E.,Nietlispach, D.,Owen, D.,Mott, H.R. (登録日: 2008-10-06, 公開日: 2008-11-11, 最終更新日: 2024-05-22)

主引用文献

Bailey, L.K.,Campbell, L.J.,Evetts, K.A.,Littlefield, K.,Rajendra, E.,Nietlispach, D.,Owen, D.,Mott, H.R.
The Structure of Binder of Arl2 (BART) Reveals a Novel G Protein Binding Domain: IMPLICATIONS FOR FUNCTION.
J.Biol.Chem., 284:992-999, 2009

PubMed Abstract: The ADP-ribosylation factor-like (Arl) family of small G proteins are involved in the regulation of diverse cellular processes. Arl2 does not appear to be membrane localized and has been implicated as a regulator of microtubule dynamics. The downstream effector for Arl2, Binder of Arl 2 (BART) has no known function but, together with Arl2, can enter mitochondria and bind the adenine nucleotide transporter. We have solved the solution structure of BART and show that it forms a novel fold composed of six alpha-helices that form three interlocking "L" shapes. Analysis of the backbone dynamics reveals that the protein is highly anisotropic and that the loops between the central helices are dynamic. The regions involved in the binding of Arl2 were mapped onto the surface of BART and are found to localize to these loop regions. BART has faces of differing charge and structural elements, which may explain how it can interact with other proteins.

PubMed: 18981177
DOI: 10.1074/jbc.M806167200

実験手法

SOLUTION NMR