2K6B

Solution structure of 1-112 fragment of human programmed cell death 5 protein

2K6B の概要

• エントリーDOI: 10.2210/pdb2k6b/pdb
• 関連するPDBエントリー: 1YYB
• NMR情報: BMRB: 15864
• 分子名称: Programmed cell death protein 5 (1 entity in total)
• 機能のキーワード: pdcd5, apoptosis, phosphoprotein
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12692.34

構造登録者

Feng, Y.,Yao, H.,Liu, D.,Wang, J. (登録日: 2008-07-07, 公開日: 2009-06-16, 最終更新日: 2024-05-29)

主引用文献

Yao, H.,Xu, L.,Feng, Y.,Liu, D.,Chen, Y.,Wang, J.
Structure-function correlation of human programmed cell death 5 protein.
Arch.Biochem.Biophys., 486:141-149, 2009

PubMed Abstract: Human programmed cell death 5 (PDCD5) is a translocatory protein playing an important role in the apoptotic process of cells. Although there are accumulated data about PDCD5 function, the correlation of the structure with the function of PDCD5 has not been investigated. Here, we report the studies of structure-function relationship of PDCD5 by multidimensional NMR methods and by FACScan flow cytometer and fluorescence microscope. The 3D structure of intact PDCD5 and the internal motions of PDCD5 have been determined. PDCD5 has a compact core structure of low flexibility with two mobile alpha-helices at N-terminal region and a flexible unstructured C-terminal region. The flow cytometry and internalization measurements of different PDCD5 fragments indicate that the charged residues are crucial for the ability of apoptosis-promoting and cell translocation of the protein. Combined analyses reveal a fact that the regions that seem to be most involved in the function also are more flexible in PDCD5.

PubMed: 19358820
DOI: 10.1016/j.abb.2009.03.018

実験手法

SOLUTION NMR