2K5O

Mouse Prion Protein (121-231) with Mutation S170N

2K5O の概要

• エントリーDOI: 10.2210/pdb2k5o/pdb
• NMR情報: BMRB: 15845
• 分子名称: Major prion protein (1 entity in total)
• 機能のキーワード: mouse prion protein, mutation, s170n, bank vole, membrane, prion, glycoprotein, golgi apparatus, gpi-anchor, hydroxylation, lipoprotein, polymorphism, unknown function
• 由来する生物種: Mus musculus (mouse)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13435.90

構造登録者

Perez, D.R.,Wuthrich, K. (登録日: 2008-06-30, 公開日: 2008-12-09, 最終更新日: 2024-10-16)

主引用文献

Christen, B.,Perez, D.R.,Hornemann, S.,Wuthrich, K.
NMR Structure of the Bank Vole Prion Protein at 20 degrees C Contains a Structured Loop of Residues 165-171
J.Mol.Biol., 383:306-312, 2008

PubMed Abstract: The recent introduction of bank vole (Clethrionomys glareolus) as an additional laboratory animal for research on prion diseases revealed an important difference when compared to the mouse and the Syrian hamster, since bank voles show a high susceptibility to infection by brain homogenates from a wide range of diseased species such as sheep, goats, and humans. In this context, we determined the NMR structure of the C-terminal globular domain of the recombinant bank vole prion protein (bvPrP) [bvPrP(121-231)] at 20 degrees C. bvPrP(121-231) has the same overall architecture as other mammalian PrPs, with three alpha-helices and an antiparallel beta-sheet, but it differs from PrP of the mouse and most other mammalian species in that the loop connecting the second beta-strand and helix alpha2 is precisely defined at 20 degrees C. This is similar to the previously described structures of elk PrP and the designed mouse PrP (mPrP) variant mPrP[S170N,N174T](121-231), whereas Syrian hamster PrP displays a structure that is in-between these limiting cases. Studies with the newly designed variant mPrP[S170N](121-231), which contains the same loop sequence as bvPrP, now also showed that the single-amino-acid substitution S170N in mPrP is sufficient for obtaining a well-defined loop, thus providing the rationale for this local structural feature in bvPrP.

PubMed: 18773909
DOI: 10.1016/j.jmb.2008.08.045

実験手法

SOLUTION NMR