2K51

NMR Solution Structure of the Neurotrypsin Kringle Domain

2K51 の概要

• エントリーDOI: 10.2210/pdb2k51/pdb
• 関連するPDBエントリー: 2K4R
• 分子名称: Neurotrypsin (1 entity in total)
• 機能のキーワード: neurotrypsin, kringle domain, disulfide-rich protein fold, serine endopeptidase, hydrolase, extracellular proteolysis
• 由来する生物種: Rattus norvegicus (Rat)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8542.46

構造登録者

Ozhogina, O.A. (登録日: 2008-06-23, 公開日: 2008-12-09, 最終更新日: 2024-10-16)

主引用文献

Ozhogina, O.A.,Grishaev, A.,Bominaar, E.L.,Llinas, M.
NMR Solution Structure of the Neurotrypsin Kringle Domain
Biochemistry, 47:12290-12298, 2008

PubMed Abstract: Neurotrypsin is a multidomain protein that serves as a brain-specific serine protease. Here we report the NMR structure of its kringle domain, NT/K. The data analysis was performed with the BACUS (Bayesian analysis of coupled unassigned spins) algorithm. This study presents the first application of BACUS to the structure determination of a 13C unenriched protein for which no prior experimental 3D structure was available. NT/K adopts the kringle fold, consisting of an antiparallel beta-sheet bridged by an overlapping pair of disulfides. The structure reveals the presence of a surface-exposed left-handed polyproline II helix that is closely packed to the core beta-structure. This feature distinguishes NT/K from other members of the kringle fold and points toward a novel functional role for a kringle domain. Functional divergence among kringle domains is discussed on the basis of their surface and electrostatic characteristics.

PubMed: 18956887
DOI: 10.1021/bi800555z

実験手法

SOLUTION NMR