2K4B

CopR Repressor Structure

2K4B の概要

• エントリーDOI: 10.2210/pdb2k4b/pdb
• NMR情報: BMRB: 15793
• 分子名称: Transcriptional regulator (1 entity in total)
• 機能のキーワード: dna binding protein, winged helix
• 由来する生物種: Lactococcus lactis subsp. lactis (Streptococcus lactis)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 11769.42

構造登録者

Cantini, F.,Banci, L.,Magnani, D.,Solioz, M. (登録日: 2008-06-03, 公開日: 2009-01-27, 最終更新日: 2024-05-29)

主引用文献

Cantini, F.,Banci, L.,Solioz, M.
The copper-responsive repressor CopR of Lactococcus lactis is a 'winged helix' protein.
Biochem.J., 417:493-499, 2009

PubMed Abstract: CopR of Lactococcus lactis is a copper-responsive repressor involved in copper homoeostasis. It controls the expression of a total of 11 genes, the CopR regulon, in a copper-dependent manner. In the absence of copper, CopR binds to the promoters of the CopR regulon. Copper releases CopR from the promoters, allowing transcription of the downstream genes to proceed. CopR binds through its N-terminal domain to a 'cop box' of consensus TACANNTGTA, which is conserved in Firmicutes. We have solved the NMR solution structure of the N-terminal DNA-binding domain of CopR. The protein fold has a winged helix structure resembling that of the BlaI repressor which regulates antibiotic resistance in Bacillus licheniformis. CopR differs from other copper-responsive repressors, and the present structure represents a novel family of copper regulators, which we propose to call the CopY family.

PubMed: 18837698
DOI: 10.1042/BJ20081713

実験手法

SOLUTION NMR