2K3V

Solution Structure of a Tetrahaem Cytochrome from Shewanella Frigidimarina

2K3V の概要

• エントリーDOI: 10.2210/pdb2k3v/pdb
• NMR情報: BMRB: 15765
• 分子名称: Tetraheme cytochrome c-type, HEME C (2 entities in total)
• 機能のキーワード: multihaem cytochromes, redox proteins, shewanella, electron transport, heme, iron, metal-binding, periplasm, transport
• 由来する生物種: Shewanella frigidimarina
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 11804.14

構造登録者

Paixao, V.B.,Turner, D.L.,Salgueiro, C.A.,Brennan, L.,Reid, G.A.,Chapman, S.K. (登録日: 2008-05-19, 公開日: 2009-03-31, 最終更新日: 2024-11-20)

主引用文献

Paixao, V.B.,Salgueiro, C.A.,Brennan, L.,Reid, G.A.,Chapman, S.K.,Turner, D.L.
The solution structure of a tetraheme cytochrome from Shewanella frigidimarina reveals a novel family structural motif
Biochemistry, 47:11973-11980, 2008

PubMed Abstract: The bacteria belonging to the genus Shewanella are facultative anaerobes that utilize a variety of terminal electron acceptors which includes soluble and insoluble metal oxides. The tetraheme c-type cytochrome isolated during anaerobic growth of Shewanella frigidimarina NCIMB400 ( Sfc) contains 86 residues and is involved in the Fe(III) reduction pathways. Although the functional properties of Sfc redox centers are quite well described, no structures are available for this protein. In this work, we report the solution structure of the reduced form of Sfc. The overall fold is completely different from those of the tetraheme cytochromes c 3 and instead has similarities with the tetraheme cytochrome recently isolated from Shewanella oneidensis ( Soc). Comparison of the tetraheme cytochromes from Shewanella shows a considerable diversity in their primary structure and heme reduction potentials, yet they have highly conserved heme geometry, as is the case for the family of tetraheme cytochromes isolated from Desulfovibrio spp.

PubMed: 18950243
DOI: 10.1021/bi801326j

実験手法

SOLUTION NMR