2K3R

Pfu Rpp21 structure and assignments

Summary for 2K3R

• Entry DOI: 10.2210/pdb2k3r/pdb
• NMR Information: BMRB: 15776
• Descriptor: Ribonuclease P protein component 4, ZINC ION (2 entities in total)
• Functional Keywords: pfu rpp21, rnase p, hydrolase, trna processing
• Biological source: Pyrococcus furiosus
• Total number of polymer chains: 1
• Total formula weight: 14987.64

Authors

Amero, C.D.,Foster, M.P.,Boomershine, W.P.,Xu, Y. (deposition date: 2008-05-15, release date: 2008-12-02, Last modification date: 2024-05-01)

Primary citation

Amero, C.D.,Boomershine, W.P.,Xu, Y.,Foster, M.
Solution structure of Pyrococcus furiosus RPP21, a component of the archaeal RNase P holoenzyme, and interactions with its RPP29 protein partner.
Biochemistry, 47:11704-11710, 2008

PubMed Abstract: RNase P is the ubiquitous ribonucleoprotein metalloenzyme responsible for cleaving the 5'-leader sequence of precursor tRNAs during their maturation. While the RNA subunit is catalytically active on its own at high monovalent and divalent ion concentrations, four protein subunits are associated with archaeal RNase P activity in vivo: RPP21, RPP29, RPP30, and POP5. These proteins have been shown to function in pairs: RPP21-RPP29 and POP5-RPP30. We have determined the solution structure of RPP21 from the hyperthermophilic archaeon Pyrococcus furiosus ( Pfu) using conventional and paramagnetic NMR techniques. Pfu RPP21 in solution consists of an unstructured N-terminus, two alpha-helices, a zinc binding motif, and an unstructured C-terminus. Moreover, we have used chemical shift perturbations to characterize the interaction of RPP21 with RPP29. The data show that the primary contact with RPP29 is localized to the two helices of RPP21. This information represents a fundamental step toward understanding structure-function relationships of the archaeal RNase P holoenzyme.

PubMed: 18922021
DOI: 10.1021/bi8015982

Experimental method

SOLUTION NMR