2K38

Cupiennin 1A, NMR, minimized average structure

2K38 の概要

• エントリーDOI: 10.2210/pdb2k38/pdb
• NMR情報: BMRB: 17015
• 分子名称: Cupiennin-1a (1 entity in total)
• 機能のキーワード: antibacterial, membrane active, amidation, antibiotic, antimicrobial, cytolysis, hemolysis, neurotoxin, secreted, helix-hinge-helix, antimicrobial protein
• 由来する生物種: Cupiennius salei (Wandering spider)
• 細胞内の位置: Secreted : P83619
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 3805.60

構造登録者

Pukala, T.L.,Boland, M.P.,Gehman, J.D.,Kuhn-Nentwig, L.,Separovic, F.,Bowie, J.H. (登録日: 2008-04-23, 公開日: 2008-05-20, 最終更新日: 2024-11-20)

主引用文献

Pukala, T.L.,Boland, M.P.,Gehman, J.D.,Kuhn-Nentwig, L.,Separovic, F.,Bowie, J.H.
Solution structure and interaction of cupiennin 1a, a spider venom peptide, with phospholipid bilayers
Biochemistry, 46:3576-3585, 2007

PubMed Abstract: The solution structure of cupiennin 1a, a 35 residue, basic antibacterial peptide isolated from the venom of the spider Cupiennius salei, has been determined by nuclear magnetic resonance (NMR) spectroscopy. The peptide was found to adopt a helix-hinge-helix structure in a membrane mimicking solvent. The hinge may play a role in allowing the amphipathic N-terminal helix and polar C-terminal helix to orient independently upon membrane binding, in order to achieve maximal antibacterial efficacy. Solid-state 31P and 2H NMR was used to further study the effects of cupiennin 1a on the dynamic properties of lipid membranes, using zwitterionic chain deuterated dimyristoylphosphatidylcholine (d54-DMPC) and anionic dimyristoylphosphatidylglycerol (DMPG) multilamellar vesicles. In d54-DMPC alone, cupiennin 1a caused a decrease in the 31P chemical shift anisotropy, indicating some interaction with the lipid head groups, and a decrease in order over the entire acyl chain. In contrast, for the mixed (d54-DMPC/DMPG) lipid system cupiennin 1a appeared to induce lateral separation of the two lipids as evidenced by the 31P spectra, in which the peptide preferentially interacted with DMPG. Little effect was observed on the deuterated acyl chain order parameters in the d54-DMPC/DMPG model membranes. Furthermore, 31P NMR relaxation measurements confirmed a differential effect on the lipid motions depending upon the membrane composition. Therefore, subtle differences are likely in the mechanism by which cupiennin 1a causes membrane lysis in either prokaryotic or eukaryotic cells, and may explain the specific spectrum of activity.

PubMed: 17319697
DOI: 10.1021/bi062306+

実験手法

SOLUTION NMR