2K1N

DNA bound structure of the N-terminal domain of AbrB

2K1N の概要

• エントリーDOI: 10.2210/pdb2k1n/pdb
• 関連するPDBエントリー: 1Z0R
• 分子名称: DNA (25-MER), AbrB family transcriptional regulator (3 entities in total)
• 機能のキーワード: abrb, abrb8, dna bound, transition state regulator, dna binding protein, activator, dna-binding, repressor, sporulation, transcription, transcription regulation, transcription-dna complex, transcription/dna
• 由来する生物種: Bacillus subtilis; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 40722.23

構造登録者

Cavanagh, J.,Bobay, B.G.,Sullivan, D.M.,Thompson, R.J. (登録日: 2008-03-10, 公開日: 2008-11-11, 最終更新日: 2024-10-16)

主引用文献

Sullivan, D.M.,Bobay, B.G.,Kojetin, D.J.,Thompson, R.J.,Rance, M.,Strauch, M.A.,Cavanagh, J.
Insights into the Nature of DNA Binding of AbrB-like Transcription Factors
Structure, 16:1702-1713, 2008

PubMed Abstract: Understanding the DNA recognition and binding by the AbrB-like family of transcriptional regulators is of significant interest since these proteins enable bacteria to elicit the appropriate response to diverse environmental stimuli. Although these "transition-state regulator" proteins have been well characterized at the genetic level, the general and specific mechanisms of DNA binding remain elusive. We present RDC-refined NMR solution structures and dynamic properties of the DNA-binding domains of three Bacillus subtilis transition-state regulators: AbrB, Abh, and SpoVT. We combined previously investigated DNase I footprinting, DNA methylation, gel-shift assays, and mutagenic and NMR studies to generate a structural model of the complex between AbrBN(55) and its cognate promoter, abrB8. These investigations have enabled us to generate a model for the specific nature of the transition-state regulator-DNA interaction, a structure that has remained elusive thus far.

PubMed: 19000822
DOI: 10.1016/j.str.2008.08.014

実験手法

SOLUTION NMR