2K0P

Determination of a Protein Structure in the Solid State from NMR Chemical Shifts

2K0P の概要

• エントリーDOI: 10.2210/pdb2k0p/pdb
• 関連するPDBエントリー: 2ju6
• 分子名称: Immunoglobulin G-binding protein G (1 entity in total)
• 機能のキーワード: solid-state, chemical shift restraints, gb1, cell wall, igg-binding protein, peptidoglycan-anchor, secreted, protein binding
• 由来する生物種: Streptococcus sp. group G
• 細胞内の位置: Secreted, cell wall; Peptidoglycan-anchor (Potential): P06654
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 6228.81

構造登録者

Robustelli, P.,Cavalli, A.,Salvatella, X.,Vendruscolo, M. (登録日: 2008-02-11, 公開日: 2009-03-03, 最終更新日: 2024-05-29)

主引用文献

Robustelli, P.,Cavalli, A.,Vendruscolo, M.
Determination of protein structures in the solid state from NMR chemical shifts.
Structure, 16:1764-1769, 2008

PubMed Abstract: Solid-state NMR spectroscopy does not require proteins to form crystalline or soluble samples and can thus be applied under a variety of conditions, including precipitates, gels, and microcrystals. It has recently been shown that NMR chemical shifts can be used to determine the structures of the native states of proteins in solution. By considering the cases of two proteins, GB1 and SH3, we provide an initial demonstration here that this type of approach can be extended to the use of solid-state NMR chemical shifts to obtain protein structures in the solid state without the need for measuring interatomic distances.

PubMed: 19081052
DOI: 10.1016/j.str.2008.10.016

実験手法

SOLID-STATE NMR