2K0C

Zinc-finger 2 of Nup153

2K0C の概要

• エントリーDOI: 10.2210/pdb2k0c/pdb
• 分子名称: Nuclear pore complex protein Nup153, ZINC ION (2 entities in total)
• 機能のキーワード: zinc-finger, dna-binding, metal-binding, mrna transport, nuclear pore complex, nucleus, phosphoprotein, protein transport, translocation, transport, metal binding protein
• 由来する生物種: Rattus norvegicus (Rat)
• 細胞内の位置: Nucleus, nuclear pore complex: P49791
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 5601.70

構造登録者

Bangert, J.A.,Schrader, N.,Vetter, I.R.,Stoll, R. (登録日: 2008-01-31, 公開日: 2008-07-01, 最終更新日: 2024-05-29)

主引用文献

Schrader, N.,Koerner, C.,Koessmeier, K.,Bangert, J.A.,Wittinghofer, A.,Stoll, R.,Vetter, I.R.
The Crystal Structure of the Ran-Nup153ZnF2 Complex: a General Ran Docking Site at the Nuclear Pore Complex.
Structure, 16:1116-1125, 2008

PubMed Abstract: Nucleoporin (Nup) 153 is a highly mobile, multifunctional, and essential nuclear pore protein. It contains four zinc finger motifs that are thought to be crucial for the regulation of transport-receptor/cargo interactions via their binding to the small guanine nucleotide binding protein, Ran. We found this interaction to be independent of the phoshorylation state of the nucleotide. Ran binds with the highest affinity to the second zinc finger motif of Nup153 (Nup153ZnF2). Here we present the crystal structure of this complex, revealing a new type of Ran-Ran interaction partner interface together with the solution structure of Nup153ZnF2. According to our complex structure, Nup153ZnF2 binding to Ran excludes the formation of a Ran-importin-beta complex. This finding suggests a local Nup153-mediated Ran reservoir at the nucleoplasmic distal ring of the nuclear pore, where nucleotide exchange may take place in a ternary Nup153-Ran-RCC1 complex, so that import complexes are efficiently terminated.

PubMed: 18611384
DOI: 10.1016/j.str.2008.03.014

実験手法

SOLUTION NMR