2K00

Solution structure of the talin F3 in complex with layilin cytodomain

Summary for 2K00

• Entry DOI: 10.2210/pdb2k00/pdb
• Descriptor: Talin-1, Layilin (2 entities in total)
• Functional Keywords: structural protein, cell projection, cytoplasm, cytoskeleton, glycoprotein, membrane, phosphoprotein, alternative splicing, lectin, transmembrane
• Biological source: Gallus gallus (Chicken); More
• Cellular location: Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side: P54939; Membrane; Single-pass type I membrane protein (Potential): Q8C351
• Total number of polymer chains: 2
• Total formula weight: 12352.12

Authors

Wegener, K.L.,Barsukov, I.L. (deposition date: 2008-01-23, release date: 2008-09-09, Last modification date: 2024-05-29)

Primary citation

Wegener, K.L.,Basran, J.,Bagshaw, C.R.,Campbell, I.D.,Roberts, G.C.,Critchley, D.R.,Barsukov, I.L.
Structural basis for the interaction between the cytoplasmic domain of the hyaluronate receptor layilin and the talin F3 subdomain
J.Mol.Biol., 382:112-126, 2008

PubMed Abstract: Talin is a large cytoskeletal protein that is involved in coupling the integrin family of cell adhesion molecules to the actin cytoskeleton, colocalising with the integrins in focal adhesions (FAs). However, at the leading edge of motile cells, talin colocalises with the hyaluronan receptor layilin in what are thought to be transient adhesions, some of which subsequently mature into more stable FAs. During this maturation process, layilin is replaced with integrins, which are highly clustered in FAs, where localised production of PI(4,5)P(2) by type 1 phosphatidyl inositol phosphate kinase type 1gamma (PIPK1gamma) is thought to play a role in FA assembly. The talin FERM F3 subdomain binds both the integrin beta-subunit cytoplasmic domain and PIPK1gamma, and these interactions are understood in detail at the atomic level. The talin F3 domain also binds to short sequences in the layilin cytoplasmic domain, and here we report the structure of the talin/layilin complex, which shows that talin binds integrins, PIPK1gamma and layilin in similar although subtly different ways. Based on structure comparisons, we designed a set of talin F3 mutations that selectively affected the affinity of talin for its targets, as determined by stopped-flow fluorescence measurements. Such mutations will help to assess the importance of the interactions between talin and its various ligands in cell adhesion and migration.

PubMed: 18638481
DOI: 10.1016/j.jmb.2008.06.087

Experimental method

SOLUTION NMR